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Peter Attia , Dr

The slides were washed in water/PBS and then blocked using 5% goat serum in PBS for 1 h at room temperature prior to adding primary antibodies diluted in the blocking buffer

The overexpression of PTEN in cancer cells increases the protein levels of all respiratory chain complexes and OXPHOS flux while also increasing the levels of mitochondrial transcription factors such as PGC1 and p53