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The peptide retains direct cytoprotective activity across the entire gastrointestinal tract, from the esophagus to the colon, derived originally from a gastric protective protein

Uba1 was PCR-amplified from cDNA and cloned into pGEX-4T1 vector, which introduced an N-terminal GST tag followed by a TEV cleavage site and a C-terminal His tag

However, the inconsistent synthesis of ALLO under conditions of hormonal instability may destabilize GABAergic signaling, promoting mood lability and increasing affective vulnerability (65,66)